NanoBRIDGES

Building bridges between specialists on computational and empirical risk assessment of engineered nanomaterials

Przemysław Oberbek (WUT) in National Institute for Environmetal Studies (NIES) in Tsukuba


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oberbek - tsukuba 2“I came back to Tsukuba with my college Marcin Wachowski. We spent 2 months (from 11 of October 2013 to 10 of December, 2013) in National Institute for Environmental Studies (NIES) working on missing data on calcium phosphates from my last visit. Year ago we characterized 12 nanosized apatite samples and conducted cell tests with them. This time we brought 18 more apatites to have enough data for nQSAR model.

Samples were characterized with Transmission Electron Microscopy (TEM) + Energy Dispersive X-ray Spectroscopy (EDS), Atomic Force Microscopy (AFM), Dynamic Light Scattering (DLS), Brunauer–Emmett–Teller method (BET) and some of them were imaged with Confocal Laser Scanning Microscopy (CLSM).

At the end of our visit, we also tried to check calcium phosphate particles interaction with hFOB 1.19 cells (human fetal osteoblastic cell line) using WST8 kit (water-soluble tetrazolium salts). Unfortunately we ran out of time to gather enough of satisfying results.

In summary, it was a great journey – both scientifically and socially. I meet a lot of scientists and gained a lot of knowledge. I would also like to continue my studies in Poland or – if there will be a chance – once again in Tsukuba.

Coordinator

University of Gdansk
Faculty of Chemistry
ul. Wita Stwosza 63
80-952 Gdansk

European Commision