Ewa Mulkiewicz in National Institute for Environmetal Studies in Tsukuba

I visited the National Institute for Environmental Studies in Tsukuba, Japan from 23.07.2012 till 18.08.2012 as a part of Work Package 2 “Carrying out experiments to produce necessary (missing) data for NPs”. During this time I evaluated suitability of NF-kappa B responsive element to study toxic potential of NPs. I treated the CHO-NF-kappa B cells with different NPs (selected dendrimers and fullerenes (both soluble and insoluble in water) and multi walled carbon nanotubes). The solubility of NPs and behaviour in conditions of in vitro experiment with the cells were tested. I also acquainted with fluorescence microscopic method as a tool for detection of reactive oxygen species (ROS) in cells exposed to chemicals in the context of future application in study of the effect of NPs to the mammalian cells.